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| Session Information |
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| Session Title: Cell Fate Speficiation - Post-Embryonic Session Type: Poster |
| Session Location: Pauley Pavilion Session Time: Sunday - Tuesday |
| Abstract Information |
| Poster Board Number: 1172A Presentation Time: SUN, June 26, 2005, 1:30-3:00PM |
| Keywords: KW41 - Methods |
| Abstract Content |
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Multi-parameter axial profiling of transgenic C. elegans
expressing fluorescent proteins from various cell-specific, tissue
specific and developmentally regulated promoters. Bo
Wang1, Julia Thompson1, Yanping Zhang2,
Michael Herman2, Mariya Lomakina1, Bruce
Holcombe1, Rock Pulak1. 1) Union Biometrica,
Somerville, MA; 2) Division of Biology, Kansas State University,
Manhattan, Kansas. The COPAS Biosort instrument automates the analysis, sorting, and dispensing of all stages of C. elegans, measuring the animal’s size and the intensity of expressed fluorescent markers. Once analyzed, animals can be selected according to user defined criteria, and then dispensed into multi-well plates for high throughput screening or collected in bulk for further analysis. With this technology, time required for large scale screening for certain changes in the optical properties of the animals, such as changes in the levels of expression of a fluorescent protein, can be dramatically reduced and human error minimized. Recent enhancements to an add-on module, called the Profiler II, have been tested for its ability to collect positional information of fluorescent expression. The instrument can simultaneously collect fluorescence information in three separate regions of the spectrum. Here we show that the instrument can analyze multi-colored transgenic animals and can be used to compare the amounts and relative positions of expression of two or three different colors of fluorescence. Furthermore, this technology can be used to screen for independent changes in the intensity or position of each reporter protein. We have tested various transgenic animals expressing green, yellow and/or red fluorescing proteins from a collection of promoters that include myo-2, str-1, egl-17, mab-5, and various others, separately and in certain combinations. We present some proof of principle examples of how these could be used in genetic screens. |
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