VAST Imagers
VAST FluoroImager Platform
Automated Positioning for Fluorescent Imaging
Fig. 1: VAST Fluoroimager System
The VAST FluoroImager automates positioning of 2-7 dpf zebrafish larvae. The larvae are oriented accurately and reproducibly within a glass capillary using a set of rotational motors. Included with the VAST FluoroImager:
- Ability to load, position, and rotate a larva to a user selected orientation
- On-board 10µm resolution camera captures precise images
- 360° imaging consisting of brightfield and 1-3 fluorescent channels
- Channel LEDs: 385nm (Violet), 470nm (Blue), 565nm (Green)
- Channel filters: 445nm, 512nm, 615nm
- Ability to generate Optical Projection Tomography (OPT) datasets with the VASTomography software
- Cellular level imaging by mounting to an upright microscope
- Seamless larva ejection and reloading from bulk reservoir
Capillary/fluorescence illumination module
Composed of the capillary unit of the VAST BioImager with the addition of the fluorescence module containing the LEDs and detectors for capturing fluorescence from the larvae.
Side View
Shows the capillary module on the right and the fluorescence illumination module to the left.
Capillary/Fluorescence Illumination Module (left) and Control Module (right)
Samples are introduced via a standard 50 ml sample cup with a gentle, suspended stirring mechanism.
FluoroImager mounted on an upright microscope
For cellular level imaging. Many different manufacturers make upright scopes that are compatible with this system.
Instrument Size
Control Module:
30 x 28 x 33 cm (depth x width x height)
Weight: 5.9 kg
Capillary/fluorescence illumination Module:
32 x 47 x 12 cm (depth x width x height)
Weight: 6 kg