Applications & Publications
Technical Notes
Developing a novel method for the screening of fungal germinated spores using hydrogel microencapsulation and large particle flow cytometry. (QTN-020)
June 18, 2013
Publications
Analysis, imaging and sorting of germinated fungal spores on the COPAS VISION Flow Cytometer
16th European Conference on Fungal Genetics
Smet et al.
February 22, 2023
Analysis, imaging and sorting of germinated fungal spores on the COPAS VISION Flow Cytometer
Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran
Gruntjes et al. May 01, 2017 https://doi.org/10.1016/j.fgb.2017.02.006
Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran
Micro-colony heterogeneity in liquid cultures
Brand Recter, Wieke Teertstra, Han Wösten March 17, 2015
View AbstractMicro-colony heterogeneity in liquid cultures
Utrecht University, Faculty of Science, Deptartment of Biology, Molecular Microbiology, Padualaan 8, 3584 CH Utrecht, The Netherlands
Flow cytometry of microencapsulated colonies for genetics analysis of filamentous fungi
Lidia Delgado-Ramos,*,†, ¹ Ana T. Marcos,*,¹ María S. Ramos-Guelfo,* Leyre Sánchez- Barrionuevo,*, Francis Smet,§, Sebastián Chávez,*, †,² and David Cánovas *,² September 19, 2014 G3: Genes|Genomes|Genetics Early Online, published on September 19, 2014 as doi:10.1534/g3.114.014357
View AbstractFlow cytometry of microencapsulated colonies for genetics analysis of filamentous fungi
*Departamento de Genética, Universidad de Sevilla, Seville, Spain
†Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío-CSIC9 Universidad de Sevilla, Seville, Spain
§Union Biometrica, Geel, Belgium
1These authors have equally contributed to this work and their names are written in alphabetical order.
2Corresponding authors
A new method for fungal genetics: flow cytometry of microencapsulated filamentous microcolonies
27th Fungal Genetics Conference
Lidia Delgado-Ramos¹, Ana T. Marcos¹, Xenia Peñate¹, María S. Ramos-Guelfo¹, Leyre Sánchez-Barrionuevo¹, Francis Smet²,
David Cánovas¹ and Sebastián Chávez¹
March 12, 2013
A new method for fungal genetics: flow cytometry of microencapsulated filamentous microcolonies
1) Departamento de Genética, Universidad de Sevilla, Spain. 2) Union Biometrica, Geel, Belgium
For more information on Cellena® which can be utilized in combination with our COPAS and BioSorter products see: www.cellena.net/en/news.html
Heterogeneity in liquid shaken cultures of Aspergillus niger inoculated with melanised conidia or conidia of pigmentation mutant
G.J. van Veluw¹, W.R. Teertstra¹, C. de Bekker¹, A. Vinck¹, N. van Beek¹, W.H. Muller², M. Arentshorst³, H.C. van der Mei4, A.F.J. Ram³, J. Dijksterhuis5, and H.A.B. Wösten¹,* September 12, 2012 Published online: 12 September 2012; doi:10.3114/sim0008. Hard copy: March 2013.
View AbstractHeterogeneity in liquid shaken cultures of Aspergillus niger inoculated with melanised conidia or conidia of pigmentation mutant
1) Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; 2) Biomolecular Imaging, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; 3) Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands; 4) Department of BioMedical Engineering, University Medical Center Groningen and University of Groningen,Hanzeplein 1, 9713 AV Groningen, The Netherlands; 5CBS-KNAW Fungal Diversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
*Correspondence: H.A.B. Wösten, h.a.b.wosten@uu.nl
Heterogeneity of Aspergillus niger in micro-colonies in Liquid Shaken Cultures.
de Bekker C, van Veluw GJ, Vinck A, Wiebenga LA, Wösten HA. February 01, 2011 Appl Environ Microbiol. Feb. 2011 Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
View AbstractHeterogeneity of Aspergillus niger in micro-colonies in Liquid Shaken Cultures.
The fungus Aspergillus niger forms (sub)millimeter microcolonies within a liquid shaken culture. Here, we show that such microcolonies are heterogeneous with respect to size and gene expression. Microcolonies of strains expressing green fluorescent protein (GFP) from the promoter of the glucoamlyase gene glaA or the ferulic acid esterase gene faeA were sorted on the basis of diameter and fluorescence using the Complex Object Parametric Analyzer and Sorter (COPAS) technology. Statistical analysis revealed that the liquid shaken culture consisted of two populations of microcolonies that differ by 90 μm in diameter. The population of small microcolonies of strains expressing GFP from the glaA or faeA promoter comprised 39% and 25% of the culture, respectively. Two populations of microcolonies could also be distinguished when the expression of GFP in these strains was analyzed. The population expressing a low level of GFP consisted of 68% and 44% of the culture, respectively. We also show that mRNA accumulation is heterogeneous within microcolonies of A. niger. Central and peripheral parts of the mycelium were isolated with laser microdissection and pressure catapulting (LMPC), and RNA from these samples was used for quantitative PCR analysis. This analysis showed that the RNA content per hypha was about 45 times higher at the periphery than in the center of the microcolony. Our data imply that the protein production of A. niger can be improved in industrial fermentations by reducing the heterogeneity within the culture.
Improved enzyme production by co-cultivation of Aspergillus niger and Aspergillus oryzae and with other fungi
H.L. Hua ¹, ², ³, J. van den Brink 4, B.S. Gruben ¹, H.A.B. Wösten ¹, 5, J.-D. Gu ², R.P. de Vries ¹, 4, 5 January 01, 2011 International Biodeterioration & Biodegradation, Volume 65, Issue 1, January 2011, Pages 248–252
View AbstractImproved enzyme production by co-cultivation of Aspergillus niger and Aspergillus oryzae and with other fungi
1 Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands 2 School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong SAR, China 3 Ministry of Agriculture Key Laboratory of Subtropical Agro-biological Disaster and Management, Fujian Agriculture and Forestry University, Fuzhou 350002, China 4 CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands 5 Kluyver Centre for Industrial Fermentation, Utrecht University, Utrecht, The Netherlands
CO-CULTIVATIONS OF FUNGI IMPROVE THE PRODUCTION OF SPECIFIC ENZYMES
ISABELLE BENOIT¹, HU HONGL͹, J. VAN DEN BRINK¹, BIRGIT GRUBEN¹, H. WOSTEN¹, R. DE VRIES²
View AbstractCO-CULTIVATIONS OF FUNGI IMPROVE THE PRODUCTION OF SPECIFIC ENZYMES
1) Microbiology,
Eurofung Meeting Hotel Excelsior Berlin, Germany October 20-21, 2011.
16th International Congress of the Hungarian Society for Microbiology, Budapest, Hungary, July 20-22, 2011.