VAST Imagers

VAST FluoroImager

Automated Positioning for Fluorescent Imaging

VAST FluoroImager System

The VAST FluoroImager automates positioning of 2-7 dpf zebrafish larvae. The larvae are oriented accurately and reproducibly within a glass capillary using a set of rotational motors. Included with the VAST FluoroImager:

• Ability to load, position, and rotate a larva to a user selected orientation
• On-board 10µm resolution camera captures precise images
• 360° imaging consisting of brightfield and 1-3 fluorescent channels
  • Channel LEDs: 385nm (Violet), 470nm (Blue), 565nm (Green)
  • Channel filters: 445nm, 512nm, 615nm
• Ability to generate Optical Projection Tomography (OPT) datasets with the VASTomography software
• Cellular level imaging by mounting to an upright microscope
• Seamless larva ejection and reloading from bulk reservoir

Capillary/Fluorescence Illumination Module (left) and Control Module (right)

Samples are introduced via a standard 50 ml sample cup with a gentle, suspended stirring mechanism.

FluoroImager mounted on an upright microscope

For cellular level imaging. Many different manufacturers make upright scopes that are compatible with this system.

Instrument Size

Control Module:
30 x 28 x 33 cm (depth x width x height)
Weight: 5.9 kg

Capillary/fluorescence illumination Module:
32 x 47 x 12 cm (depth x width x height)
Weight: 6 kg