COPAS Flow Cytometers
COPAS Infinity Cytometers
Increased sensitivity in a smaller footprint
Introducing the most recent addition to the COPAS family – COPAS Infinity. Designed to accommodate the evolving needs of the modern lab, this platform combines a smaller footprint, aerosol containment, UV sterilization, sorting and high sensitivity optics with all the proven capabilities of COPAS cytometers. COPAS Infinity is sized for installation in a biosafety cabinet for biocontainment and possess expanded capabilities for sample analysis and sorting. As on all COPAS instruments, the range of sample sizes is accommodated by four configurations with different flow cell sizes. COPAS Infinity-250, Infinity-500, Infinity-1000, and Infinity-2000 can accommodate samples ranging from 2 to 1500 microns in diameter across this product.
COPAS Infinity Large Particle Flow Cytometer with Sorting
The COPAS Infinity platform is designed for the measurement of objects ranging in size from 2-1500 microns in diameter. Each of the four COPAS Infinity models features an optimized fluidic path, flow cell and optics engineered for a specific subset of the size range delivering maximum sensitivity, speed, accuracy and throughput. Instruments can dispense objects either into bulk receptacles or multiwell plates.
Each COPAS Infinity system can be equipped with up to three lasers. Six optical detectors measure up to 31 different fundamental parameters per each object in a sample. PIN diode detectors measure size, optical density and forward scattered light. Four PMT detectors measure side scatter and fluorescence emissions.
The real-time analysis of these measured parameters is used to make sort decisions. Only those objects meeting the user selectable sort criteria are gently dispensed into multiwell plates or tubes. The sorting chamber is enclosed providing aerosol containment and UV sterilization to enhance biosafety and sterility. Those objects not meeting the sort criteria are diverted by a puff of air to a sample recovery container where they may be recaptured, unharmed and viable.
Instruments are equipped with an X–Y stage to permit the dispensing of objects into collection tubes and multiwell plates, including 24-, 48-, 96-, and 384-well plate formats. Using the FlowPilot™ software, users may custom design templates for additional plate formats. All systems are supplied with a Windows® workstation running FlowPilot™ software for operation and analysis.
Choosing a COPAS Infinity Model
Each COPAS Infinity system features a specially engineered fluidic path and flow cell optimized for a specific object size range to achieve the highest accuracy and sensitivity possible. The table shown at Choosing the Right Platform and Model summarizes the largest object size recommended for each COPAS Infinity model and also provides general system recommendations based on some sample applications. The applications listed are not exhaustive but simply meant to provide a general overview of system capabilities. For a more detailed discussion of your particular application, including those applications that are not listed here, please contact our applications scientists directly at AppSupport@unionbio.com.
Infinity-250
The COPAS Infinity-250 instrument features our smallest flow cell available (250 µm) and is ideal for use with all stages of C.elegans and other small organisms, large cells like mature adipocytes, cardiomyocytes, hepatocytes, cell clusters like ESC’s / EB’s, hepatocytes, pancreatic islets, and duct cells, as well as small beads and seeds with sizes from 2 to 200 microns in diameter. Although the Infinity-500 can also be used for sorting C. elegans, the Infinity-250 is preferable if you need to sort eggs as well as adults. The specially designed fluidic path and small flow cell have been optimized for these small objects and provide the highest size resolution measurements, the smallest sorting dispense volumes, and achieves the fastest sorting rates.
Infinity-500
The COPAS Infinity-500 model features a mid-size flow cell (500 µm) for use with all stages of C. elegans, Drosophila embryos to first instar larvae, pollen, beads and seeds, various cell clusters and organoids. The ideal sample object diameter for this instrument is between 30 and 350 microns but objects ranging from 6 to 400 microns in diameter can also be run making this one of our most versatile systems, ideal for use by multiple labs with varied applications.
Infinity-1000
The COPAS Infinity-1000 instrument features a larger-size flow cell (1000 µm) for use with Drosophila embryos to second instar larvae, Arabidopsis seeds, mosquitoes, beads, cell clusters, organoids and hydrogel encapsulated cells. The ideal sample object diameter for this instrument is between 30 and 750 microns but objects ranging from 15 to 850 microns in diameter can be run providing a flexible system suitable for use by multiple labs with varied applications.
Infinity-2000
This system features our largest flow cell to date (2000 µm) and has been specially designed for use with early third instar Drosophila larvae, zebrafish embryos, beads, cell clusters and organoids. The ideal sample object diameter for this instrument is between 100 and 1400 microns but objects ranging, from 40 to 1500 microns in diameter can be run. This system is ideal for sorting large objects into tubes, bulk containers or multiwell plates up to the 96-well format.