About Union Biometrica
Nematode Sort by Size and Fluorescence
As quantifying fluorescence by conventional means is relatively time consuming, we also used a new technique that relies on an automated nematode sorter that can measure the size and fluorescence of each individual nematode in a population. We used the COPAS (complex object parametric analysis and sorting) Biosort to quantify the effect of tir-1 RNAi on the constitutive and infection-induced expression of both the pnlp-31::gfp and pnlp-29::gfp reporter genes.