Publications

Flow Cytometry Publications - Bacteria

Identification and Characterisation of a pH-stable GFP

Tania M. Roberts, Fabian Rudolf, Andreas Meyer, Rene Pellaux, Ellis Whitehead, Sven Panke, and Martin Held
June 21, 2016
Scientific Reports, (2016) London: Nature Publishing Group.

Aggregation of germlings is a major contributing factor towards mycelial heterogeneity of Streptomyces.

Zacchetti et al.
May 31, 2016
Sci Rep. 2016 May 31;6:27045. doi: 10.1038/srep27045.

Optimization of a whole-cell biocatalyst by employing genetically encoded product sensors inside nanolitre reactors

Andreas Meyer¹,², René Pellaux¹,², Sébastien Potot³, Katja Becker¹, Hans-Peter Hohmann³, Sven Panke¹ & Martin Held¹
July 13, 2015
Nature Chemistry 7, 673–678 (2015) doi:10.1038/nchem.2301

1Department of Biosystems Science and Engineering, ETH Zurich, Basel 4058,

2FGen GmbH, Basel 4057, Switzerland
 
3DSM Nutritional Products, Kaiseraugst 4303, Switzerland

Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

Marloes L. C. Petrus¹, G. Jerre van Veluw², Han A. B. Wösten², Dennis Claessen¹
February 13, 2014
J. Vis. Exp. (84), e51178, doi:10.3791/51178 (2014).

1)Microbial Biotechnology, Institute Biology Leiden, Leiden University, 2)Microbiology, Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University

Analysis of two distinct mycelial populations in liquid-grown Streptomyces cultures using a flow cytometry-based proteomics approach.

van Veluw GJ, Petrus ML, Gubbens J, de Graaf R, de Jong IP, van Wezel GP, Wösten HA, Claessen D.
October 16, 2012
Appl Microbiol Biotechnol. 2012 Oct 16. [Epub ahead of print]

Department of Microbiology, Institute of Biomembranes, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, The Netherlands.

NOVEL HIGH THROUGHPUT TECHNOLOGIES FOR BACTERIAL LIBRARY SCREENING IN LIFESCIENCE

MARCEL WALSER
May 31, 2010
DISS. ETH NO. 19176

ETH Zurich

Novel method for high-throughput colony PCR screening in nanoliter-reactors

Marcel Walser¹, Rene Pellaux¹, Andreas Meyer¹, Matthias Bechtold¹, Herve Vanderschuren², Richard Reinhardt³, Joseph Magyar4, Sven Panke¹ and Martin Held¹,*
February 26, 2009
Nucl. Acids Res. (2009) 37 (8): e57/1-e57/8

1) ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), 2) ETH Zurich, Institute of Plant Science, Plant Biotechnology, Zurich, Switzerland, 3) Max Planck Institute for Molecular Genetics, Analytics & Computing, Berlin, Germany and 4) Harlan Laboratories Ltd., Environmental Safety and Metabolism, Itingen, Switzerland

Isolation of monoclonal microcarriers colonized by fluorescent E. coli

Walser M, Leibundgut RM, Pellaux R, Panke S, Held M.
June 16, 2008
ETH Zurich, Institute of Process Engineering, BioProcess Laboratory, Universitätsstr. 6/CAB H88, CH-8092 Zurich, Switzerland.

Microencapsulation can be used for high-throughput (HT) processing of bacterial libraries. Ideally, each microcarrier would contain exactly one cell or colony. However, synthesis yields a mixture of capsules containing the desired one cell,  multiple cells and empty microcarriers. E. coli cells expressing green fluorescent protein (GFP) were encapsulated in 35 nl Hydrogel carriers. HT sorting procedures by a COAPS sorter were then used to enrich samples to 95% monoclonality.

Published Online: Jun 16 2008 2:41PM; DOI: 10.1002/cyto.a.20597; Cytometry A. 2008 Sep;73(9):788-98.